diff --git a/tests/qtest/libqos/riscv-iommu.h b/tests/qtest/libqos/riscv-iommu.h
index d123efb41f..318db13799 100644
--- a/tests/qtest/libqos/riscv-iommu.h
+++ b/tests/qtest/libqos/riscv-iommu.h
@@ -62,6 +62,36 @@
 
 #define RISCV_IOMMU_REG_IPSR            0x0054
 
+#define RISCV_IOMMU_REG_IVEC            0x02F8
+#define RISCV_IOMMU_REG_IVEC_CIV        GENMASK_ULL(3, 0)
+#define RISCV_IOMMU_REG_IVEC_FIV        GENMASK_ULL(7, 4)
+#define RISCV_IOMMU_REG_IVEC_PMIV       GENMASK_ULL(11, 8)
+#define RISCV_IOMMU_REG_IVEC_PIV        GENMASK_ULL(15, 12)
+
+#define RISCV_IOMMU_REG_CQB             0x0018
+#define RISCV_IOMMU_CQB_PPN_START       10
+#define RISCV_IOMMU_CQB_PPN_LEN         44
+#define RISCV_IOMMU_CQB_LOG2SZ_START    0
+#define RISCV_IOMMU_CQB_LOG2SZ_LEN      5
+
+#define RISCV_IOMMU_REG_CQT             0x0024
+
+#define RISCV_IOMMU_REG_FQB             0x0028
+#define RISCV_IOMMU_FQB_PPN_START       10
+#define RISCV_IOMMU_FQB_PPN_LEN         44
+#define RISCV_IOMMU_FQB_LOG2SZ_START    0
+#define RISCV_IOMMU_FQB_LOG2SZ_LEN      5
+
+#define RISCV_IOMMU_REG_FQT             0x0034
+
+#define RISCV_IOMMU_REG_PQB             0x0038
+#define RISCV_IOMMU_PQB_PPN_START       10
+#define RISCV_IOMMU_PQB_PPN_LEN         44
+#define RISCV_IOMMU_PQB_LOG2SZ_START    0
+#define RISCV_IOMMU_PQB_LOG2SZ_LEN      5
+
+#define RISCV_IOMMU_REG_PQT             0x0044
+
 typedef struct QRISCVIOMMU {
     QOSGraphObject obj;
     QPCIDevice dev;
diff --git a/tests/qtest/riscv-iommu-test.c b/tests/qtest/riscv-iommu-test.c
index 7f0dbd0211..c38a0a160d 100644
--- a/tests/qtest/riscv-iommu-test.c
+++ b/tests/qtest/riscv-iommu-test.c
@@ -33,6 +33,20 @@ static uint64_t riscv_iommu_read_reg64(QRISCVIOMMU *r_iommu, int reg_offset)
     return reg;
 }
 
+static void riscv_iommu_write_reg32(QRISCVIOMMU *r_iommu, int reg_offset,
+                                    uint32_t val)
+{
+    qpci_memwrite(&r_iommu->dev, r_iommu->reg_bar, reg_offset,
+                  &val, sizeof(val));
+}
+
+static void riscv_iommu_write_reg64(QRISCVIOMMU *r_iommu, int reg_offset,
+                                    uint64_t val)
+{
+    qpci_memwrite(&r_iommu->dev, r_iommu->reg_bar, reg_offset,
+                  &val, sizeof(val));
+}
+
 static void test_pci_config(void *obj, void *data, QGuestAllocator *t_alloc)
 {
     QRISCVIOMMU *r_iommu = obj;
@@ -84,10 +98,123 @@ static void test_reg_reset(void *obj, void *data, QGuestAllocator *t_alloc)
     g_assert_cmpuint(reg, ==, 0);
 }
 
+/*
+ * Common timeout-based poll for CQCSR, FQCSR and PQCSR. All
+ * their ON bits are mapped as RISCV_IOMMU_QUEUE_ACTIVE (16),
+ */
+static void qtest_wait_for_queue_active(QRISCVIOMMU *r_iommu,
+                                        uint32_t queue_csr)
+{
+    QTestState *qts = global_qtest;
+    guint64 timeout_us = 2 * 1000 * 1000;
+    gint64 start_time = g_get_monotonic_time();
+    uint32_t reg;
+
+    for (;;) {
+        qtest_clock_step(qts, 100);
+
+        reg = riscv_iommu_read_reg32(r_iommu, queue_csr);
+        if (reg & RISCV_IOMMU_QUEUE_ACTIVE) {
+            break;
+        }
+        g_assert(g_get_monotonic_time() - start_time <= timeout_us);
+    }
+}
+
+/*
+ * Goes through the queue activation procedures of chapter 6.2,
+ * "Guidelines for initialization", of the RISCV-IOMMU spec.
+ */
+static void test_iommu_init_queues(void *obj, void *data,
+                                   QGuestAllocator *t_alloc)
+{
+    QRISCVIOMMU *r_iommu = obj;
+    uint64_t reg64, q_addr;
+    uint32_t reg;
+    int k = 2;
+
+    reg64 = riscv_iommu_read_reg64(r_iommu, RISCV_IOMMU_REG_CAP);
+    g_assert_cmpuint(reg64 & RISCV_IOMMU_CAP_VERSION, ==, 0x10);
+
+    /*
+     * Program the command queue. Write 0xF to civ, fiv, pmiv and
+     * piv. With the current PCI device impl we expect 2 writable
+     * bits for each (k = 2) since we have N = 4 total vectors (2^k).
+     */
+    riscv_iommu_write_reg32(r_iommu, RISCV_IOMMU_REG_IVEC, 0xFFFF);
+    reg = riscv_iommu_read_reg32(r_iommu, RISCV_IOMMU_REG_IVEC);
+    g_assert_cmpuint(reg & RISCV_IOMMU_REG_IVEC_CIV, ==, 0x3);
+    g_assert_cmpuint(reg & RISCV_IOMMU_REG_IVEC_FIV, ==, 0x30);
+    g_assert_cmpuint(reg & RISCV_IOMMU_REG_IVEC_PMIV, ==, 0x300);
+    g_assert_cmpuint(reg & RISCV_IOMMU_REG_IVEC_PIV, ==, 0x3000);
+
+    /* Alloc a 4*16 bytes buffer and use it to set cqb */
+    q_addr = guest_alloc(t_alloc, 4 * 16);
+    reg64 = 0;
+    deposit64(reg64, RISCV_IOMMU_CQB_PPN_START,
+              RISCV_IOMMU_CQB_PPN_LEN, q_addr);
+    deposit64(reg64, RISCV_IOMMU_CQB_LOG2SZ_START,
+              RISCV_IOMMU_CQB_LOG2SZ_LEN, k - 1);
+    riscv_iommu_write_reg64(r_iommu, RISCV_IOMMU_REG_CQB, reg64);
+
+    /* cqt = 0, cqcsr.cqen = 1, poll cqcsr.cqon until it reads 1 */
+    riscv_iommu_write_reg32(r_iommu, RISCV_IOMMU_REG_CQT, 0);
+
+    reg = riscv_iommu_read_reg32(r_iommu, RISCV_IOMMU_REG_CQCSR);
+    reg |= RISCV_IOMMU_CQCSR_CQEN;
+    riscv_iommu_write_reg32(r_iommu, RISCV_IOMMU_REG_CQCSR, reg);
+
+    qtest_wait_for_queue_active(r_iommu, RISCV_IOMMU_REG_CQCSR);
+
+    /*
+     * Program the fault queue. Alloc a 4*32 bytes (instead of 4*16)
+     * buffer and use it to set fqb.
+     */
+    q_addr = guest_alloc(t_alloc, 4 * 32);
+    reg64 = 0;
+    deposit64(reg64, RISCV_IOMMU_FQB_PPN_START,
+              RISCV_IOMMU_FQB_PPN_LEN, q_addr);
+    deposit64(reg64, RISCV_IOMMU_FQB_LOG2SZ_START,
+              RISCV_IOMMU_FQB_LOG2SZ_LEN, k - 1);
+    riscv_iommu_write_reg64(r_iommu, RISCV_IOMMU_REG_FQB, reg64);
+
+    /* fqt = 0, fqcsr.fqen = 1, poll fqcsr.fqon until it reads 1 */
+    riscv_iommu_write_reg32(r_iommu, RISCV_IOMMU_REG_FQT, 0);
+
+    reg = riscv_iommu_read_reg32(r_iommu, RISCV_IOMMU_REG_FQCSR);
+    reg |= RISCV_IOMMU_FQCSR_FQEN;
+    riscv_iommu_write_reg32(r_iommu, RISCV_IOMMU_REG_FQCSR, reg);
+
+    qtest_wait_for_queue_active(r_iommu, RISCV_IOMMU_REG_FQCSR);
+
+    /*
+     * Program the page-request queue. Alloc a 4*16 bytes buffer
+     * and use it to set pqb.
+     */
+    q_addr = guest_alloc(t_alloc, 4 * 16);
+    reg64 = 0;
+    deposit64(reg64, RISCV_IOMMU_PQB_PPN_START,
+              RISCV_IOMMU_PQB_PPN_LEN, q_addr);
+    deposit64(reg64, RISCV_IOMMU_PQB_LOG2SZ_START,
+              RISCV_IOMMU_PQB_LOG2SZ_LEN, k - 1);
+    riscv_iommu_write_reg64(r_iommu, RISCV_IOMMU_REG_PQB, reg64);
+
+    /* pqt = 0, pqcsr.pqen = 1, poll pqcsr.pqon until it reads 1 */
+    riscv_iommu_write_reg32(r_iommu, RISCV_IOMMU_REG_PQT, 0);
+
+    reg = riscv_iommu_read_reg32(r_iommu, RISCV_IOMMU_REG_PQCSR);
+    reg |= RISCV_IOMMU_PQCSR_PQEN;
+    riscv_iommu_write_reg32(r_iommu, RISCV_IOMMU_REG_PQCSR, reg);
+
+    qtest_wait_for_queue_active(r_iommu, RISCV_IOMMU_REG_PQCSR);
+}
+
 static void register_riscv_iommu_test(void)
 {
     qos_add_test("pci_config", "riscv-iommu-pci", test_pci_config, NULL);
     qos_add_test("reg_reset", "riscv-iommu-pci", test_reg_reset, NULL);
+    qos_add_test("iommu_init_queues", "riscv-iommu-pci",
+                 test_iommu_init_queues, NULL);
 }
 
 libqos_init(register_riscv_iommu_test);
